琼脂糖凝胶与DNA浓度
琼脂糖凝胶与DNA 浓度
胶浓度(w/v) 理想分离范围 理想的电泳液
0.8% 800-22,000 bp TAE
1.0% 500-10,000 bp TAE/TBE
1.2% 400-7,000 bp TAE/TBE
1.5% 250-5000 bp TAE/TBE
2.0% 150-3,000 bp TBE
The genomic DNA was dissolved in TE buffer [10 mM Tris (pH 7.4), 10 mM EDTA (pH 8.0)] and analyzed by gel electrophoresis at 50 V for 90 min using 2 % agarose. Approximately 20μg of genomic DNA was loaded in each well, visualized under ultraviolet (UV) light and photographed.
基因组DNA （RNase A处理过）2%琼脂糖凝胶，50V ，90min 。DNA 量约20μg。
Fig. 3 DNA fragmentation induced in A549 cells which treated with piperine for 48 h (lane amarker, laneb200μg/ml, lane c100μg/ml, laned50μg/ml, lane f control)
Yi Lin, Jianping Xu, Hehe Liao, Lu Li, Lei Pan. Piperine induces apoptosis of lung cancer A549 cellsvia p53-dependent mitochondrial signaling pathway. Tumor Biol. (2014) 35:3305–3310.